【摘 要】
：
Objective To prepare doxorubicin-loaded hollow organosilica nanosphere (DOX-HON) and to investigate the cell toxicity and drug-release properties and the efficacy for treating metastatic hormone-refra
【机 构】
：
Department of Ultrasound in Medicine,Shanghai Jiao Tong University Affiliated 6th Hospital,Shanghai
【出 处】
：
第三届全国暨国际超声分子影像及生物效应和治疗学术会议
论文部分内容阅读
Objective To prepare doxorubicin-loaded hollow organosilica nanosphere (DOX-HON) and to investigate the cell toxicity and drug-release properties and the efficacy for treating metastatic hormone-refractory prostate cancer (HRPC) of the DOX-HONs in vitro.Methods The cell toxicity of the DOX-HONs for PC3 lines were evaluated with MTS.The DOX loading efficacy,loading on the HONs, and pH responsive release characteristics were determined utilizing UV-Visible spectroscopy.The effects of the DOX-HON on HRPC cell line PC3 were evaluated with MTS and live/dead cell viability assays.Results Cell viability was 84% without serum and 95% with serum when the concentration of HON was 100μg/ml.Cell viability was 19% without serum and 40% with serum when the concentration of HON was 300μg/ml.DOX-HON displayed exceptional loading efficiency(60%) and drug loading on HONs(37.5 wt%) with optimal release at pH 4.5(85%).In comparison to treatment with DOX alone, cell death significantly increased when cells were treated with DOX-HON complexes.Of the tested treatments,DOX-HON with 2.5μg/ml DOX exhibited superior efficacy (55% cell death).DOX-HON with 1.25μg/rnl DOX achieved 37% cell death, which was comparable to cell death in response to 2.5μg/ml of free DOX,suggesting that HONs aid in decreasing the DOX dose necessary to achieve a chemotherapeutic efficacy.Conclusion Due to its enhanced efficacy, DOX-HON can be used to successfully treat HRPC and potentially decrease the clinical side effects of DOX.
其他文献
随着纳米生物与肿瘤微/无创诊疗技术的快速发展，一种具有温敏响应性质的无机基纳米超声诊疗剂材料由于具有良好的热/化学稳定性、易于表面修饰与结构/尺寸调控及避免血池造影等优点，引起了国内外研究者的极大关注。通过功能化改性与结构设计，可进一步与核磁共振和光声成像等形成多模式成像，并实现与化疗和光热疗等协同高效治疗肿瘤。本研究面向超声肿瘤诊疗领域的实际应用，围绕更安全、更高效治疗肿瘤的关键科学问题，重点介
For more than 25 years, Bracco is involved in the research and development of ultrasound contrast agents (comprising gaseous microbubbles).Braccos commitment to providing improved diagnostic imaging s
定量超声方法检测与评价骨质及诊断骨质疏松症是近年来医学超声领域内的研究热点之一，并已取得了显著的进展.超声背散射信号比透射信号能够提供更多的关于松质骨微结构的信息.报告将介绍骨超声背散射检测与评价骨质的原理和方法，并介绍一种新型超声背散射骨质诊断仪及其在1226例成人和467例新生儿骨检测中的应用.在中心频率3.5 MHz和5.0 MHz下，测量并计算表观积分背散射(AIB)、频谱质心偏移(SCS
胰腺癌的恶性程度极高,患者的五年生存率不足5％,其特点是显著的间质纤维化反应.近年来,人们逐渐认识到肿瘤间质微环境对胰腺癌的发展起着至关重要的作用.胰腺星形细胞是胰腺间质的主要成分,在间质纤维化的发生发展中扮演了重要角色.肿瘤的进展与转移会伴随着丰富的血管生成以满足其代谢需要,胰腺癌的生长亦依赖于血管供应营养物质.Periostin基因最初在小鼠胚胎成骨细胞MC3T3-E1中发现,命名为成骨细胞特
医学影像学是现代医学发展最快的学科之一，已从传统的解剖成像进入了分子显像与治疗时代。随着多功能超声分子探针和超声非线性成像技术的不断发展与完善，以及一些新技术如光声成像、光热治疗等技术的出现，使得超声分子显像不仅能在细胞或分子水平早期诊断疾病，又能在分子成像监控下进行靶向治疗或药物及载基因定位传递，实现疾病的早期诊断与精准治疗一体化。目前，虽已取得了一些令人鼓舞的进展，但亦存在诸多技术难点、面临许
目的 通过联合超声靶向破碎微泡技术(UTMD)与脂质体/目的基因质粒DNA/核定位信号(PEI/DNA/NLS)复合物构建新型基因转导体系,并评价该体系转染293T细胞的转染效率.方法 用琼脂糖凝胶试验检测PEI/DNA/NLS复合物是否构建成功以及PEI/NLS对质粒DNA是否存在保护作用.用超声辐照仪对含有质粒pEGFP-DNA、PEI/DNA复合物,PEI/DNA/NLS复合物,SonoVu
目的 研究纳米泡的显像能力和在肿瘤组织中的穿透力,同时制备携载针对前列腺特异性膜抗原(prostate specific membrane antigen,PSMA)的适体分子A10-3.2的靶向超声纳米泡,并研究其在诊断前列腺癌中的作用.方法 将一定比例的脂质材料在水合液中进行机械震荡,并采用离心漂浮法分离超声纳米泡,并在动物体内观察纳米泡注射前后彩色血流信号的变化情况.在光镜及共聚焦显微镜下观
目的 研究制备的载抗ICAM-1单抗的靶向微泡造影剂在体外及体内的靶向能力.方法 构建ICAM-1质粒,对其进行扩增以及提取之后检测提取质粒的浓度、进行酶切电泳并对构建质粒进行测序；体外培养Hela细胞,脂质体转染法将ICAM-1质粒转染进细胞,使Hela细胞表达ICAM-1表面抗原；并在显微镜下观察转染成功后Hela细胞与靶向微泡的结合情况.通过对家兔左侧跟腱注射Ⅰ型胶原酶制造家兔跟腱模型,另一
Objective Photoacoustic imaging (PAI) is a non-invasive and nonionizing imaging modality relying on the photoacoustic effect.It is a dual imaging modality that overcomes the depth limitation of the cu
Objective To prepare polymeric vesicles having uniform size,high drug loading capacity and ultrasound sensitivity through an unusual method of pH-induced micelle-to-vesicle conversion (PMVC) using tri