Dissecting the gene regulation network of human early embryos at single cell and single base resolut

• 作 者: FuchouTang[1]SunneyXie[1]JieQiao[2]
• 机 构: Biodynamic Optical Imaging Center,College of Life Sciences,Peking University,Beijing 100871,P.R.Chin
• 出 处: 遗传学与表观遗传学前沿暨第三届中国青年遗传学家论坛
• 发表日期: 2014年期

　　Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development.Here we apply single-cell RNA sequencing (RNA-Seq) analysis to 124 individual cells from human preimplantation embryos and human embryonic stem ceils (hESCs) at different passages.The number of maternally expressed genes detected in our data set is 22,687, including 8,701 long noncoding RNA s (lncRNA s), which represents a significant increase from 9,735 maternal genes detected previously by cDNA microarray.We discovered 2,733 novel lncRNA s, many of which are expressed in specific developmental stages.To address the long-standing question whether gene expression signatures of human epiblast (EPI) and in vitro hESCs are the same, we found that EPI cells and primary hESC outgrowth have dramatically different transcriptomes, with 1,498 genes showing differential expression between them.This work provides a comprehensive framework of the transcriptome landscapes of human early embryos and hESCs.