Development and validation of a multiplex reverse transcription PCR assay for simultaneous detection

• 作 者: DecaiTuo;WentaoShen;YongYang;PuYan;XiaoyingLi;PengZhou;
• 机 构: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture,Institute
• 出 处: 2014年度中国热带作物学会遗传育种专业委员会/热带香料饮料作物专业委员会年会暨学术研讨会
• 发表日期: 2014年期

　　Papaya ringspot virus (PRSV), Papaya leaf distortion mosaic virus (PLDMV), and Papaya mosaic virus (PapMV) produce similar symptoms in papaya.Each threatens commercial production of papaya on Hainan Island, China.In this study, a multiplex reverse transcription PCR assay was developed to detect simultaneously these three viruses by screening combinations of mixed primer pairs and optimizing the multiplex RT-PCR reaction conditions.A mixture of three specific primer pairs was used to amplify three distinct fragments of 613 bp from the P3 gene of PRSV, 355 bp from the CP gene of PLDMV, and 205 bp from the CP gene of PapMV,demonstrating the assays specificity.