The characteristic of Multi-tracers uptake in hepatocellular carcinoma of different metastatic abili

• 作 者: He Tingting Zhang Jin ming Tian Jiahe Zhang Xiaojun Liu Jian Wang Hui Chang Zhen
• 机 构: Nuclear Medicine Department,The Genera/Hospital of the Chinese People's Liberation Army,Beijing 100
• 出 处: The 1st Sino-American Conference on Nuclear Medicine(首届中美核医学
• 发表日期: 2011年12期
• 关键词: positron emission tomography 18F-FDG 18F-fluroaceret 18F-FLT hepatocellular carc

　　Objective: The aim of the study was to evaluate whether the multi-tracers (18F-FDG, 18F-FAC and 18F-FLT) PET imaging could differentiate and reflect the metastatic potential of hepatocellular carcinoma (HCC).Methods: Human HCC cell lines, MHCC97-H and MHCC97-L, with different metastatic potential, were cultured and implanted into nude mice.Invasion and meatastasis ability were assessed by Matrigel invasion assay in vitro and Spontaneous metastasis assay in vivo.In vivo imaging of tumor-bearing mice was obtained by animal PET/CT scan at 60min after injection of different kinds of tracers (18F-FDG, 18F-FAC and 18F-FLT) respectively.Protein expression and mRNA level of CD44 (a marker for metastasis character) was detected by Western blot and real-time PCR.The correlation between uptake of tracers and CD44 were performed by correlation analysis.Results: In vitro, MHCC97-H cells exhibited a higher level of Matrigel invasion than MHCC97-L cells.In vivo, spontaneous metastasis assay revealed that 83.3% (5/6) lymph node metastasis was found in MHCC97-H model, and 50% (3/6) in MHCC97-L.In Micro-PET study, the uptake ratio (T/NT, tumor/non-tumor) of 18F-FDG and 18F-FAC in MHCC97-H tumor-bearing models were higher than that in MHCC97-L models (18F-FDG: P<0.05, 18F-FAC: P<0.05).Whereas, the 18F-FLT uptake in MHCC97-H tumor-bearing model was significantly lower than that in MHCC97-L model (P<0.05).The expression level of CD44 in MHCC97-H tumor was higher than that in MHCC97-L.And 18F-FAC and 18F-FLT uptake was correlated with CD44 mRNA level (18F-FAC: P<0.05, 18F-FLT: P<0.05).But there was no significantly correlation between 18F-FDG uptake and CD44 mRNA level (P>0.05).Conclusions: Metastatic potential of MHCC97-H tumor was higher than MHCC97-L tumor.Combining of 18F-FDG, 18F-FAC and 18F-FLT PET imaging could predict different metastasis ability of HCC tumors.