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Acetate has a strong inhibitory effect on hydrogen production[1-3].We developed an integrated system taking electrodialysis with bipolar membrane as separation unit coupling with an anaerobic bioreactor[4].Batch teats of hydrogen production were carried out.The pH of fermentation broth increased exponentially with BPEM operation.Glucose spreaded into concentrating chamber resulting in 3%glucose losing.The integrated system effectively released the acetate inhibition,elevated the hydrogen producing capacity and enhanced the stability of the whole process.