论文部分内容阅读
The impact of mercury stress on Venerupis philippinarum gill and haemocytes was assessed through investigating the dynamic changes of several biochemistry parameters,including superoxide dismutase(SOD),catalase(CAT),glutathione reductase(GR),acid phosphatase(ACP),alkaline phosphatase(ALP),total glutathione content,as well as malondialdehyde(MDA)content,under different Hg2+ acute exposure in the present study.Results showed that SOD activities and GSH contents in the gill displayed depressed expression.The correlation analysis indicated significant positive correlations of the two parameters(0.833,P < 0.01).GR showed elevated activities in all examined exposure doses.While the activities of CAT and ALP were only induced at higher Hg2+ concentrations with significant positive correlations(0.917,P < 0.01),and MDA showed decreased contents in middle exposure doses.In term of haemocytes,Hg exposure had an induced effect on the activities of CAT,GR,ACP and ALP,and MDA content increased with increasing concentration of Hg at 24 h.All these parameters had significant positive correlations(P < 0.01).No obvious dose-dependent or time-dependent expression profiles were detected in other indexes in both tissues.All these results showed that acute Hg exposure led to harmful effects on clams immune system,which are likely linked to Hg induced oxidative stress.CAT,ACP,and MDA in gill have great potential as biomarkers of Hg concentration determination in the aquatic environment for the studied species.