【摘 要】
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Background Nowadays, cancer is one of the most main causes leading to death, which seriously endangers human health and survival.Due to the photo thermal anticancer activity, carbon materials such as
【机 构】
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School of Pharmaceutical Sciences, Zhengzhou University, Henan, P.R.China
【出 处】
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第二届全国生物颗粒学术研讨会、第三届国际工业药学和临床药学研讨会暨第一届岭南国际药学大会
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Background Nowadays, cancer is one of the most main causes leading to death, which seriously endangers human health and survival.Due to the photo thermal anticancer activity, carbon materials such as grapheme oxide, fullerene and carbon nanotubes, have become commonly used load carriers of anticancer drugs.Objective this study was to prepare and characterize the carriers which were modified by hyaluronic acid (HA-GO, HA-C60, HA-SWNT);in addition, the photo thermal anticancer activity of these carriers was compared in vitro.The distribution of these carriers was studied in vivo.Method We synthesized hyaluronic acid (HA) with a specific targeting property to conjugate with grapheme oxide, fullerene or carbon nanotubes by inserting ethylenediamine as spacer.Their morphology was investigated by transmission electron microscopy (TEM).The thermal efficiency in vitro was evaluated with infrared camera.The cell inhibition rate of HA-ethylenediamine-carbon material conjugates was measured by SRB assay using MCF-7 cells.The distribution of these carriers was investigated by in vivo imaging analysis.Results Their morphology showed these carbon materials with hyaluronic acid modified were rougher than those without hyaluronic acid modified, which demonstrated that these carbon materials were successfully modified by hyaluronic acid with ethylenediamine as spacer (Fig1).The thermal efficiency in vitro assay showed that thermal efficiency of HA-SWNT was the highest(from 28.0℃ to 90.7℃),HA-GO was lower(from 27.0℃ to 50.4℃),while there was almost no change with HA-C60(from 27.9℃ to 33.0℃)(Fig2).The SRB assay denoted that in the same concentration, HA-SWNT exhibited the strongest inhibitory action, HA-GO followed, the last was HA-C60(Fig3).Compared with the free IR783,these carriers loaded with IR783 showed the specific tumor-targeting capacity(Fig4).Conclusion The results of in vitro comparison demonstrated that among the highest photothermal anticancer activity, HA-SWNT occupied first place;HA-GO was second;HA-C60 was last.Moreover, they expressed specific tumor-targeting in vivo.
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