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There is need to detect and control the cancer at an early stage by developing the newer targets and newer agents.Epigenetics / methylation precede neoplasia and if they are responsive to anticancer agents they could be used as early signatures for the assessment or detection of cancer.The present study deals with the status of epigenetic changes for the detection and control of tumor-before it is developed.The study was carried out using ethylnitosourea (ENU),a tumorigen, in mouse model of lung tumor development in presence or absence of inositol hexaphosphate (IP6), an antitumor agent.Hyperplasia and lymphocytic infiltrations were observed at three months but tumors were not there.However, they were present at eight months after the exposure.Molecular analysis was conducted to catch hold of the early changes in the three months exposed samples.Tumor suppressor gene, p 16, was down regulated by ENU at the level of protein and mRNA both.IP6 tried to revert this down regulation.ENU also down regulated the global DNA methylation and up regulated the p 16 gene promoter CpG methylation.Thereafter, we observed the up regulation of the regulatory events of epigenetic machinery in terms of the expressions of DNMT1, MeCP2, MBD 1 and HDAC 1 after the tumorigen exposure.IP6 exhibited the protection at all the levels.We could observe the altered epigenetic events before we could observe the tumors.The pathway leading to gene promoter CpG methylation that affects the gene expression appeared to be involved in the development and progression of tumors.We can say that there are some epigenetic changes which are altered when the tumor starts developing and anticancer agents (IP6) modulate the gene expression through these changes.Hence, they can be considered for the detailed study for the timely management of the tumor development and also can be exploited for the development of newer anti cancer agents.