【摘 要】
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Dendrobium officinale (Orchidaceae) is an endangered plant species with important medicinal value.To evaluate the effectiveness of ex situ collection of D.officinale genetic diversity,we developed 15
【机 构】
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Nanjing Normal University,China
【出 处】
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世界自然保护联盟(IUCN)兰花专家组亚洲委员会成立大会暨第八届亚洲兰花多样性与保育国际学术研讨会
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Dendrobium officinale (Orchidaceae) is an endangered plant species with important medicinal value.To evaluate the effectiveness of ex situ collection of D.officinale genetic diversity,we developed 15 polymorphic trinucleotide microsatellite loci of D.officinale to examine the genetic diversity and structure of three D.officinale germplasm collections comprising 120 individuals from its germplasm collection base and their respective wild populations consisting of 62individuals from three provinces in China.The three germplasm collections showed reductions in gene diversity and average number of alleles per locus,but an increase in average number of rare alleles (frequency≤0.05) per locus in comparison to their wild populations.However,the differences in gene diversity between the germplasm collections and wild populations were not statistically significant.The analysis using STRUCTURE revealed evident differences in genetic composition between each germplasm collection and its wild population,probably because the D.officinale individuals with distinct genotypes in each wild population were unevenly selected for establishing its germplasm collection.For conservation management plans,we propose that D.officinale individuals with rare alleles need to be conserved with top priority,and those individuals with the most common alleles also should be concerned.The 15 new microsatellite loci may be used as a powerful tool for further evaluation and conservation of the genetic diversity of D.officinale germplasm resources.
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