Cleavage of cytochrome c1 by the porcine reproductive and respiratory syndrome virus 3C-like proteas

• 作 者: Feng Zhang Xinna Ge Peng Gao Lei Zhou Xin Guo Jun Han Hanchun Yang
• 机 构: Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture, College of Veteri
• 出 处: 2016第六届中国兽药大会(中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016学术年会、中国微生物学会兽医微
• 发表日期: 2016年12期

　　A growing number of positive-stranded RNA viruses actively induce cell apoptosis to facilitate virus spread and to evade host immunity, but the molecular mechanisms are poorly understood.In this report, we show that the nonstructural protein 4 (nsp4), 3C-like protease of porcine reproductive and respiratory syndrome virus (PRRSV) interacts with the mitochondrial inner membrane protein cytochrome c 1 (cyto.c 1) and induces its proteolytic cleavage.Importantly,the cleaved N-terminal fragment of cyto.cl is a strong apoptosis inducer that causes mitochondrial fragmentation, leading to cell apoptosis.Further RNAi silencing experiments reveal that cyto.cl is critical for PRRSV-induced apoptosis.Taken together, the PRRSV 3C-like protease mediated cleavage of cyto.c 1 is a critical trigger of cell apoptosis.