【摘 要】
：
For recent progress in sequencing technology,DNA sequencing is more efficiently and economically and in greater depth than ever before.One key challenge for gen
【机 构】
：
NationalKeyLaboratoryofCropGeneticImprovement,HuazhongAgriculturalUniversity,Wuhan,Hubei430070,China
论文部分内容阅读
For recent progress in sequencing technology,DNA sequencing is more efficiently and economically and in greater depth than ever before.One key challenge for genome sequencing project is assembling scaffolds into high-quality chromosome-scale scaffolds.Recently,some studies established an effective and efficient solution to the challenge of chromosome-scale scaffolds assembly by chromatin interaction data.In this study,we assessed the feasibility and validity of the chromatin interaction-based de novo assembly in plants using Arabidopsis thaliana ecotype Landsberg erecta(Ler)as an example.We performed high-throughput chromosome conformation capture(HiC)technology analysis on Ler and obtained 102,232,785 paired reads.Then,we build a de novo assembly for the Ler with 1,705 scaffolds by clustering scaffolds to chromosome groups,ordering scaffolds within chromosome group and assigning relative orientations to individual scaffolds.We found that the assembly results presented a high consistency with homology to a reference genome(Col)and a previous assembly using a high resolution genetic map generated in Ler.Our study demonstrated the capability of this method to assemble the scaffolds in plant accurately and efficiency.The development of de novo chromosome-scale plant genome assembly based on chromatin interactions creates new opportunities for building complete and accurate reference sequences and conducting precise and rigid analyses of unexplored genomes in a very cost-effective way,opening the door to numerous substantial new analyses in plants.
其他文献
Fruits are unique to flowering plants and play a central role in seed maturation and dispersal.Molecular dissection of fruit ripening has received considera
Fruit ripening in Actinidia sp(kiwifruit)is complex,and atypical.Many of the fruit ripening associated processes(starch breakdown,flesh colour change,and in
As the physiology of the fruit changes during maturation and senescence,the inhibitors of fungal growth decreased and the fruit became more susceptible to p
The primary aim of this study was to understand the cross-fertilize compatibility and the xenia effect of different pollens from different pollinizers in K
Turkey is the leading country with 795.000 tons/year of fresh apricot production in total world production of about 3.9 million tons.Half of the 16 million
Fluorescence microscopy is a relatively rapid and reliable method to determine serf-incompatibility in fruit tree species.It is based on observation of poll
This study,lasting two years,was carried out to determine prevalence status,contamination level,parasitoids and predators of Sphaerolecanium prunastri in Ma
The New Zealand apricot industry has opportunities to expand exports to Asia.Current cultivars may not be sweet enough for Asian consumers because soluble s
Fresh apricot(Prunus armeniaca L.)was one of the most popular fruits in ancient times when fruit were harvested close to or at eating-ripe and sold locally.
In apricot(Prunus armeniaca),fruit quality at consumption and shelf-life potential are strictly related to the ripening stage at harvest.As a results the de