论文部分内容阅读
目的:建立HPLC法测定人血浆中右旋酮洛芬的浓度。方法:血浆样品以乙醚为萃取剂,萘普生为内标物;采用Shim-pack VP-ODS色谱柱(150 mm×4．6 mm,5μm);流动相为乙腈-5 mmol·L-1磷酸缓冲液(22:78,pH=6．8),流速1．0 ml·min-1;紫外检测波长262 nm。结果:右旋酮洛芬血浆样品的线性范围为0．02-20．00μg·ml-1,r=0．999 9,n=8。萃取回收率和方法回收率分别为72．28％-75．63％和104．0％-107．8％(n=5),日内、日间RSD分别为0．6％-6．2％和2．7％-4．8％(n=5)。结论:本法简便,准确,重现性好。
Objective: To establish a HPLC method for the determination of dexketoprofen in human plasma. METHODS: Plasma samples were extracted with diethyl ether and naproxen was used as internal standard. Shim-pack VP-ODS column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-5 mmol·L -1 Phosphate buffer (22:78, pH = 6.8), flow rate 1.0 ml · min-1; UV detection wavelength 262 nm. Results: The linear range of dexketoprofen plasma samples was 0.02-20.00 μg · ml-1, r = 0.999 9, n = 8. The extraction recovery rate and recovery rate were 72.28% -75.63% and 104.0% -107.8% (n = 5) respectively. The intra-day and inter-day RSD were 0.6% -6.2% And 2.7% -4.8% (n = 5). Conclusion: This method is simple, accurate and reproducible.