Increased osteogenesis in osteoporotic bone marrow stromal cells by the overexpression of leptin

• 作 者: FuhuaYan[1]Baoyuzheng[2]KaiLuo[2]
• 机 构: Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, Jiangsu, China
• 出 处: 2014全国口腔生物医学学术年会暨“西湖国际”口腔医学高峰论坛
• 发表日期: 2014年期

　　Osteoporosis leads to increased bone fractures and net bone loss, in part because of dysfunctional bone marrow stromal cells (BMSCs).Leptin is an adipokine that plays important roles in many biological processes, including regulating the actions of mesenchymal stem cells.The aim of this study was to investigate the osteogenic effects of leptin in osteoporotic BMSCs in vitro and in vivo.The leptin gene was transferred into BMSCs isolated from osteoporotic rats by using recombinant adenoviruses.After the gene and protein expressions of leptin confirming, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were used to assess the effect of leptin on cell viability.