3T3-L1细胞诱导分化成熟阶段2种脂肪细胞因子的表达水平研究

来源 :第七届中国畜牧科技论坛 | 被引量 : 0次 | 上传用户：haojian19831212

【摘要】

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【作者】

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潘红梅闫尊强秦先红谢玉容陈磊

【机构】

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重庆市畜牧科学院,重庆荣昌,402460 甘肃农业大学动物科学技术学院,甘肃兰州,730070

【出处】

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第七届中国畜牧科技论坛

【发表日期】

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2016年4期

【关键词】

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脂肪细胞细胞分化增强子结合蛋白α 脂联素基因表达

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本研究采用诱导分化培养法诱导小鼠3T3-L1前脂肪细胞分化为脂肪细胞.油红O染色法检测脂肪细胞的脂滴积累与脂肪细胞分化程度,发现在诱导分化后的7、8、9天能够观察到明显的脂质沉积,脂滴大小和数量均表现出逐渐增加的趋势.荧光定量PCR对增强子结合蛋白α(C/EBPα)和脂联素(ADIPOQ)两种脂肪细胞因子的基因相对表达量进行检测,结果发现在小鼠3T3-L1前脂肪细胞的分化过程中,随分化时间的延长,C/EBPα和ADIPOQ两个基因在3T3-L1前脂肪细胞的脂质高度累积阶段呈现出不同的表达量变化趋势.说明在小鼠3T3-L1前脂肪细胞分化为成熟脂肪细胞的过程中,C/EBPα对3T3-L1前脂肪细胞的分化和脂质积累可能起促进作用,而AdipoQ基因对3T3-L1细胞的诱导分化可能有促进作用,但与细胞内脂质的累积关系不大.

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3T3-L1细胞诱导分化成熟阶段2种脂肪细胞因子的表达水平研究

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