寄生疫霉菌小RNA调控寄主植物基因表达的初步研究

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户：hujieguang

【摘要】

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【作者】

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贾津布王秦虎单卫星

【机构】

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旱区作物逆境生物学国家重点实验室,杨凌712100;西北农林科技大学植物保护学院,杨凌712100

【出处】

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中国植物病理学会2014年学术年会

【发表日期】

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2014年3期

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　　寄生疫霉菌是一种寄主范围广泛的根部土传病原菌,是重要的模式卵菌,在生产上造成大量的经济损失。小RNA是真核生物普遍存在的重要的调节因子,为了探索寄生疫霉菌小RNA能否进入植物细胞,并干扰寄主植物的免疫反应和新陈代谢,我们以GFP为报告基因检测寄生疫霉菌小RNA进入寄主植物潜力。稳定表达GFP-dsRNA的寄生疫霉菌菌株(GPK菌株)能够产生大量GFP-siRNA,并且能够侵染稳定表达GFP的拟南芥和烟草的根部和叶部。寄生疫霉菌侵染24h的转GFP烟草根部和侵染72h的转GFP拟南芥根部的荧光均比接菌前减弱,但相对于野生型菌株侵染的转GFP的烟草和拟南芥,GPK菌株侵染的植物GFP荧光强度变化动态没有明显差异。

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