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Objective:To assess the therapeutic effect of treating diabetic foot ulcers (DFUs) in rats by transplanting subcutaneously around the wounds and intramuscularly into the leg with bone marrow derived mesenchymal stem cells (BM-MSCs).Methods:BM-MSCs from male Wistar rats were cultured by the whole bone marrow adherence method until the third generation.Then BM-MSCs were labeled by 4,6-diamino-2-phenylindole (DAPI) in vitro.