Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplo

• 作 者: JinfengZhao[1]KaiZhao[2]ZuogangPeng[1]
• 机 构: Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education),Southwest Uni
• 出 处: 中国海洋湖沼学会、中国动物学会鱼类学分会第九届会员代表大会暨2014年学术研讨会
• 发表日期: 2014年期

　　Triplophysa rosa is an endangered cave-dwelling and endemic fish species found only in Chongqing,southwestern China.The genetic data available for this species is very limited.Polymorphic microsatellites were identified in the genome of T.rosa using 454 sequencing.Of the 145 loci screened,106 were amplified successfully and 11 showed polymorphic patterns.The number of alleles ranged from 2 to 6,and the observed and expected heterozygosity varied from 0.061 to 0.543 (mean=0.349) and from 0.248 to 0.789 (mean=0.551),respectively.The polymorphic information content (PIC) ranged from 0.215 to 0.744 (mean=0.486),indicating moderate levels of polymorphism.In addition,cross-species amplification was tested for the 106 loci in Triplophysa moquensis,which showed a high level of transferability (76.4%),implying that the microsatellite markers developed here could be used effectively for other closely related species.