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The pomegranate dry rot(Zythia versoniana Sacc.) is an important disease of pomegranate in worldwide.The disease severely occurs especially in China.Integrated control system has been used for the control of the disease, in which chemotherapy plays an important role at present.The hemotherapy-dominant control system brought many problems to the environment, such as chemical residua in the products and induction of resistance to fungicide(s) by the pathogen.To solve these problems, an intensive study on biocontrol of pomegranate dry rot is needed.Isolations and characterizations of biocontrol agents are the basis for biocontrol of the disease.A bacterial strain Z2 with strong inhibiting ability against Zythia versoniana Sacc., was isolated from pomegranate orchard soil in Zaozhuang city, China.The isolated bacterial strain was identified and characterized as Bacillus subtilis on the base of its morphology and 16S rDNA sequence analysis as well as physiological and biochemical characters.The results of dual-culture method indicated the inhibition rate of Z2 strain between 66.7%~71.1%.The results showed that Z2 has potential as biocontrol agent against Pomegranate dry rot.