【摘 要】
:
目的 探讨小鼠骨髓间充质干细胞(mBMSCs)各亚群在心肌修复中抗凋亡能力的比较.方法 以小鼠心肌干细胞表面分化抗原检测mBMSCs后以CD45、CD31为标准分选得到小鼠骨髓间充质干细胞四个亚群.体外将四个亚群采用无血清低氧(5%)培养,流式检测各组的凋亡率.分别将细胞注入心梗48小时小鼠体内注射后48小时完成心功能测定.
【机 构】
:
云南省第一人民医院心脏大血管外科 650032
【出 处】
:
第17届中国南方国际心血管病学术会议
论文部分内容阅读
目的 探讨小鼠骨髓间充质干细胞(mBMSCs)各亚群在心肌修复中抗凋亡能力的比较.方法 以小鼠心肌干细胞表面分化抗原检测mBMSCs后以CD45、CD31为标准分选得到小鼠骨髓间充质干细胞四个亚群.体外将四个亚群采用无血清低氧(5%)培养,流式检测各组的凋亡率.分别将细胞注入心梗48小时小鼠体内注射后48小时完成心功能测定.
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