PP2A (PR65) in silver carp cDNA clone and expression analysis

• 作 者: Junguo Ma Xiaoyu Li
• 机 构: CollegeofLifeScience,HenanNormalUniversity,Xinxiang,Henan453007,China
• 出 处: 中国毒理学会生化与分子毒理专业委员会学术会议暨环境内分泌干扰物的毒理学研究新技术新方法研讨会
• 发表日期: 2014年10期
• 关键词: PP2A-A silver carp cDNA clone expression microcystins

　　In the present study, the full-length sequence of protein phosphatases 2A subunit A (PP2A-A) cDNA was cloned, sequenced, and the constitutive expression of PP2A-A was also analyzed in silver carp.Moreover, the transcription of PP2A-A in the fish liver, kidney, and spleen after microcystins (MCs)-exposure were also determined in this study.The results reveal that the full length ofPP24-A cDNA is 2 833 base pair (bp) long and contains an open reading frame of 1 767 bp encoding a protein of 589 amino acids with an estimated molecular weight of 65.47 kDa and an isoelectric point of 4.95.Sequence analysis reveals that PP2A is highly conserved in fish.Furthermore, the results of quantitative real-time PCR reveal that PP2A-A is constitutively expressed in all tissues of silver carp, but mainly in brain, liver and heart.In addition, we also found that 50 or 200 μg kg-1 of crude MCs exposure remarkably down-regulated the transcription of PP2A-4 in the liver, kidney, and spleen of silver carp, suggesting that PP2A-A may be involved in MC-hepatotoxicity in silver carp.