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Flash NanoPrecipitation(FNP)is an easy scalable and fast processing method for preparing of nanoparticles(NPs)with a simply vortex equipment.By using the FNP method,fluorescent nanoparticles are prepared less than 1 second in a multi-inlet vortex mixer,in which hydrophobic aggregation-induced emission(AIE)-active dye of EDP is incorporated within the biocompatible block copolymer PEG-b-PCL for EDP-NPs assembly.The formulation parameters of stream velocity,dyes as well as loading and concentration in FNP are optimized.The sizes of NPs ranged from 20 to 60 nm with the ratio change of mixed solvents.