Investigation on diversity and population succession dynamics of indigenous bacteria in maize spermo

来源 :中国作物学会作物种子专业委员会2013年学术年会 | 被引量 : 0次 | 上传用户:zqtoo
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The spermosphere as an important habitat in plant micro-ecosystem has a·inique significance of seed microbial ecology,while the related reports has been scarce.In this study,the mature seeds of reciprocal cross maize (Zea mays L.,Nongda108) were collected to investigate the diversity and population succession dynamics of spermosphere indigenous bacteria in 12 h,24 h and 36 h of seeds germination by 16S rDNA library construction.It was found that the spermosphere indigenous bacteria of Nongda108 included Pseudomonas、Burkholderia、Bacillus、Paenibacillus、Stenotrophomonas and so on.In the spermosphere of one plant sample of the reciprocal cross maize,Nongda108A (Huang C×178),the first and second dominant bacteria genera identified were Pseudomonas and Burkholderia respectively,In the time course of seeds germination,the kinds of d0minant genera of Nongda108A were not changed,and with seeds germinating,the abundance of Pseudomonas was increased with the order of 59.60%,75.00% and 82.61%; while Burkholderia was decreased with the order of 39.39%,25.00% and 15.22%.The kinds of dominant bacteria genera of Nongda108B (178×Huang C) changed among the time course of seeds germination.Bacillus、Paenibacillus and Stenotrophomonas as the dominant genera from the other plant sample, Nongda108B.The population succession dynamies of indigenous bacteria in Nongda108B was more apparent than the other.The abundance of Paenibacillus as the same dominant genus after 12h,24h and 36h of seeds germination was decreased with the order of 68.00%,46.15% and 13.27%; while the abundance of Stenotrophomonas (32.00%)、Bacillus (53.85%) and Burkholderia (77.55%) as dominant genera after 12 h,24 h and 36 h increased respectively.Some dominant bacteria genera identified from maize spermosphere were the common PGPR.
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