The study of continuous microwave assisted protein enzymatic digestion

• 作 者: Zhengyi Chen Meiping Wei Rihui Su Guihua Ruan
• 机 构: College of Chemistry and Bioengineering,Guilin University of Technology,Guilin,541004,China
• 出 处: 第19届全国色谱学术报告会
• 发表日期: 2013年11期
• 关键词: Microwave assisted digestion immobilization Trypsin

　　Several recent reports [1-2] have highlighted the speed and convenience of microwave-assisted enzymatic digestion.However,the microwave usually could be weakened when it rose at presupposed temperature of researchers,which leaded to the neglect of the effect of microwave.Here we use a condensing device to dissipate the heat so that the circumstance of digestion can stay in the stable temperature and the full power of microwave is maintained.Immobilized trypsin and bovine serum albumin(BSA)were as enzyme and substrate respectively.The whole device is shown as Figure1.