Differential expression of sigma and omega glutathione S-transferases from Ruditapes philippinarum t

• 作 者: LinbaoZhang[1]XiaoliLiu[2]ChenghuaLi[2]AiqinChen[2]JianminZhao[2]HuifengWu;
• 机 构: Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai,264003,China
• 出 处: 中国科学院“百人学者论坛”2010年学术年会
• 发表日期: 2010年期

　　Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics,and also play important roles in antioxidant defense.In this study,two glutathione S-transferase isoforms (RpGSTS,sigma GST; RpGSTO,omega GST) were cloned from Manila clam (Ruditapes philippinarum) by RACE approaches.The expression profiles of RpGSTS and RpGSTO responded to heavy metals and benzo[a]pyrene exposure were investigated by quantitative real-time RT-PCR.The expression of RpGSTS and RpGSTO were both rapidly up-regulated,however,they showed different expression patterns to different toxicants.The expression level of RpGSTS in haemolymph with Cd exposure increased much higher than that of RpGSTO,while there existed similar expression patterns for both RpGSTS and RpGSTO from Cu exposed group.For B[α]P exposure,the maximum induction of RpGSTO was approximately two times higher than that of RpGSTS.Taken together,RpGSTS exhibited to be potentially a sensitive biomarker for Cd exposure,while RpGSTO could serve as a marker of B[α]P in environmental monitoring programs.