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Purpose: To determine the function of hsa-miR-663 in the HeLa cells responding to radiation and tumor proliferation.Materials and methods: The precursor of hsa-miR-663 was cloned into pcDNATM6.2-GW/EmGFP-miR and the expression cassette was transferred into pT-REx-DEST30 which contains a tetracycline operator through gateway reaction.Stable inducible hsa-miR-663 expressing cell line, which is named HeLa-TetR-663, was constructed by transfecting HeLa cells stably expressing tetracycline repressor (TetR) with pT-REx-DEST30-663 and selected with G418 for 14 days.After verification of hsa-miR-663 expression by qRT-PCR, the HeLa-TetR-663 cells were injected subcutaneously into the flanks of NOD/SCID mice (n=5 flanks).After formation of tumors, the miR-663 expression was induced continuously for 7 days by injecting tetracycline subcutaneously once a day.Then the tumors were subjected to 5 Gy X-Rays and the tumor volume was assessed every 2 days post-irradiation using a caliper.Results: The tumor growth of radiation group as well as radiation plus induction group is slower than that of control.However, the induction group obviously exhibited high tumor growth rate.Conclusions: Hsa-miR-663 functions as an oncogene and its high expression promote the tumor growth in vivo.