The peiminine stimulating autophagy in human colorectal carcinoma cells via AMPK pathway by SQSTM1

• 作 者: Zhi Zheng Qinsi He Liting Xu Wenhao Cui Hua Bai Zhe Zhang Jun Rao Fangfang Dou
• 机 构: Graduate School of Medicine, Nanchang University, Nanchang, Jiangxi, 330029, P.R.China;Jiangxi Cance
• 出 处: 第六届中国肿瘤绿色治疗新技术论坛、第四届老年肿瘤内科规范化诊治及新进展研讨会
• 发表日期: 2016年8期
• 关键词: peiminine autophagy natural product autophagic cell death SQTEM1 AMPK/mTOC/ULK s

　　Autophagy is a conserved catabolic process,which functions in maintenance of cellular homeostasis in eukaryotic cells.The self-eating process engulfs cellular long-lived proteins and organelles with double-membrane vesicles,and forms a so-called autophagosome.Degradation of contents via fusion with lysosome provides recycled building blocks for synthesis of new molecules during stress,e.g.starvation.Peiminine is a steroidal alkaloids extracted from Fritillaria thunbergii which is widely used in Traditional Chinese Medicine.Previously,peiminine has been identified to induce autophagy in human colorectal carcinoma cells.In this study,we further investigated that peiminine could induce autophagic cell death via activating autophagy-related signaling pathway AMPK-mTOR-ULK by promoting SQSTMl(P62).Xenograft tumor growth in vivo suggested that both peiminine and starvation inhibit the growth of tumor in size and weight,which was prominently enhanced when peiminine and starvation combined.The therapeutical effect of peiminine in cancer treatment is to be expected.