【摘 要】
：
研究目的：从冷水虹鳟鱼肠道黏膜筛选抗感染益生菌株,并进行体内外抗感染免疫验证.研究方法及结果：本研究从辽宁省本溪山区半野生健康虹鳟幼鱼的肠道黏膜中分离了68株土著菌
【出 处】
：
中国免疫学会第十届全国免疫学学术大会
论文部分内容阅读
研究目的：从冷水虹鳟鱼肠道黏膜筛选抗感染益生菌株,并进行体内外抗感染免疫验证.研究方法及结果：本研究从辽宁省本溪山区半野生健康虹鳟幼鱼的肠道黏膜中分离了68株土著菌株,首先对虹鳟68株土著菌株进行体外免疫学初步评价和筛选,即运用TNF-αactivated HT29-cells and PBMCs System体外细胞模型对候选菌株进行体外筛选,结果获得了6株具有体外刺激相应细胞产生IL-8和IL-12p70/IL-10比值较高的菌株(P＜0.05),其中一株为芽孢杆菌HZC58株(菌株保藏编号为：CGMCC No.10706)；其次进行了体外抗病毒验证,芽孢杆菌HZC58菌株与EPC细胞(鱼上皮细胞)共培养,结果芽孢杆菌HZC58菌株相互作用的实验组细胞上清病毒效价为107.7TCID50,对照组细胞上清病毒效价为108.5TCID50,统计分析表明差异极显著(P＜0.01)；再次进行了虹鳟鱼口服芽孢杆菌HZC58菌株的体内免疫学评价,结果表明：口服实验组脾脏的IL-1β含量极显著高于PBS对照组,差异极显著(P＜0.0001)；口服实验组血清的TNF-α含量、IL-1β含量、IgM含量极显著高于对照组,差异极显著(P＜0.0001)；口服实验组血清的总补体ACH50、SOD含量均显著高于对照组,差异极显著(P＜0.01).
其他文献
IL-25 is an important immune regulator that can promote Th2 immune response-dependent immunity, inflammation,and tissue repair in asthma, intestinal infecti
Background: Enterovirus 71 (EV71) is a major pathogen for severe hand, foot, and mouth disease (HFMD), which has high morbidity and mortality.
The human T cell leukemia virus type 1 (HTLV-1) is a complex human retrovirus that causes an aggressive leukemia known as adult T cell leukemia (ATL).
Background: The host Zinc finger antiviral protein (ZAP) has been reported exhibiting antiviral activity against positive-stranded RNA viruses (Togaviridae)
Aim: Hepatitis C virus (HCV) infection is characterized by a strong propensity toward chronicity.During chronic HCV infection, HCV core protein is implicate
研究背景：HBV感染已成为世界性难题。中国是HBV感染高发区，慢性HBV感染人数为全球总数的1/3左右。随着感染周期延长，多数患者因肝癌等终末期肝病而死亡。因此HBV感染是需及
Background: Encephalomyocarditis virus (EMCV) is the causative agent of myocarditis, diabetes and reproductive and nervous system disorders in many mammalia
CXCL8是一种炎性趋化因子,由多种细胞产生,可引起中性粒细胞趋化,导致其变性及脱颗粒,许多研究已证实,在感染、创伤、缺氧、缺血以及自身免疫性疾病的患者可检测出高水平
Background and Objective: Osteopontin (OPN) expression is increased during the course of various chronic inflammatory diseases, including tuberculosis (TB).
研究背景：解脲脲原体(Ureaplasma urealyticum,Uu)是性传播和垂直传播病原体,血清型3和14是常见感染型别。多带抗原(Multiple banded antigen,MBA)是Uu主要外膜蛋白,为群