金针菇多糖对肝癌HepG2细胞增殖抑制及凋亡诱导作用研究

来源 :2016全国多糖研讨会 | 被引量 : 0次 | 上传用户：angelgsj

【摘要】

：

　　目的：研究金针菇水提多糖(FVP)对肝癌HepG2细胞增殖抑制及凋亡诱导作用方法：热水浸提金针菇,75％乙醇沉淀得到FVP,MTT 法检测不同浓度FVP 处理HepG2细胞24h、48h 后增殖抑制

【作者】

：

张爱龙甄东苗月任桂红宋慧

【机构】

：

吉林农业大学生命科学学院

【出处】

：

2016全国多糖研讨会

【发表日期】

：

2016年期

【关键词】

：

金针菇多糖肝癌 HepG2细胞增殖抑制细胞凋亡细胞形态变化 FVP

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

　　目的：研究金针菇水提多糖(FVP)对肝癌HepG2细胞增殖抑制及凋亡诱导作用方法：热水浸提金针菇,75％乙醇沉淀得到FVP,MTT 法检测不同浓度FVP 处理HepG2细胞24h、48h 后增殖抑制效果,以正常肝细胞L02 为阴性对照。筛选最佳处理浓度及时间。用Annexin V-FITC/PI 细胞凋亡试剂盒检测细胞凋亡情况,Hoechst33258 染色法观察细胞形态变化。

其他文献

PHOTO-DRIVEN CONTROLLED RADICAL POLYMERIZATION FOR GRADIENT NANODOMAIN FORMATION

"Photo-induced" controlled radical polymerization has gained increased attention to advance well-known controlled radical polymerization techniques such as nitr

会议

SYNTHESIS OF NEW POLYAMIDES STARTING FROM CYCLIC LYSINE OR BY MARRYING ANIONIC RING-OPENING POLYMERI

会议

HPLC CHARACTERIZATION AND PURIFICATION OF CYCLIC POLYMERS

Cyclic polymers have attracted much attention owing to their unique topology-related physical properties.Such studies have been accelerated in recent years than

会议

ENZYMATIC RING-CLOSURE OF MACROCYCLES AND RING-OPENING POLYMERIZATION FOR POLYESTERS

Previously we prepared macrocyclic monomers and then polyesters through Ru-catalyzed ring closure and opening metathesis reactions.It is rather difficult to rid

会议

SYNTHESIS OF POLYMERS WITH CONTROLED DEGRDATION VIA INTRAMOLECULAR CYCLIZATION

Polymers which undergo controlled degradation in response to external stimulus such as pH,light,redox or oxidation conditions,temperature and so on have attract

会议

ADVANCED SYNTHESIS OF POLYPEPTIDES

(1) The ring-opening polymerization (ROP) of amino acid N-carboxyanhydride (NCA) is initiated by a mixture of primary ammonium salt and tertiary amine.

会议

POLYMERIZATIONS WITH ELEMENTAL SULFURA ROUTE TO IMPROVED NEXT GENERATION BATTERIES

The development of chemistry and processing methods for the utilization of elemental sulfur into polymeric materials provides a unique opportunity for addressin

会议

SEQUENCE CONTROLLED COPOLYMERIZATION VIA COORDINATION MECHANISM

As elegantly expressed by the nature that the DNA-based biomacromolecules accomplishing the life-secret code is attributed to the strictly arranged aminaacid al

会议

SILYL KETENE POLYMERIZATION

The applications of polymers are dictated by their properties which are in turn reliant on not only the processing conditions and overall polymer molecular weig

会议

NUCLEOPHILIC THIOL-YNE CLICK CHEMISTRYFOR THE SYNTHESIS OF TOUGH MATERIALS

The application of click chemistry has enabled the facile synthesis and functionalization of a range of materials on account of the orthogonal and efficient nat

会议

金针菇多糖对肝癌HepG2细胞增殖抑制及凋亡诱导作用研究

其他学术论文