【摘 要】
：
目的：调查四川省猪肉源大肠杆菌污染情况,了解菌株对抗生素及季铵盐类消毒剂(QACs)的耐药情况.方法：从四川省各市县收集猪肉样品130份,选择性培养基分离大肠杆菌后VITEK进行鉴定；采用K-B法(CLSI)药敏试验测试大肠杆菌对10种药敏纸片的耐药性；琼脂稀释法测定大肠杆菌对三种季铵盐消毒剂的MIC值,PCR扩增10种季铵盐类消毒剂耐药基因.结果：130份猪肉样品中分离大肠杆菌96株,分离率为7
【机 构】
：
四川农业大学都江堰校区微生物学实验室,都江堰,611830
【出 处】
：
中国食品科学技术学会第十届年会暨第七届中美食品业高层论坛
论文部分内容阅读
目的：调查四川省猪肉源大肠杆菌污染情况,了解菌株对抗生素及季铵盐类消毒剂(QACs)的耐药情况.方法：从四川省各市县收集猪肉样品130份,选择性培养基分离大肠杆菌后VITEK进行鉴定；采用K-B法(CLSI)药敏试验测试大肠杆菌对10种药敏纸片的耐药性；琼脂稀释法测定大肠杆菌对三种季铵盐消毒剂的MIC值,PCR扩增10种季铵盐类消毒剂耐药基因.结果：130份猪肉样品中分离大肠杆菌96株,分离率为73.85％；其中73株对抗生素产生耐药,耐药率分别为：TET(64.58％)、AMP(37.50％)、S(32.29％)、K(21.88％)、KF(20.83％)、CIP(18.75％)、CN(9.38％)、SAM(6.25％)、CAE(2.08％)、CRO(2.08％),共产生了28种耐药谱,TET是最主要的谱型；96株大肠杆菌对季铵盐消毒剂BC、DDAC、CTAB的MIC分别为：16-64mg/L、8-32mg/L、64-256mg/L； QACs耐药基因检出率分别为ydgE/F(81.25％)、mdfA(50％)、sugE(45.83％)、emrE(36.46％)、 qacE△1(19.79％)、qac F(17.71％)、qacE(14.58％)、SugE2(3.13％),qac G-未检出.讨论：四川省肉源大肠杆菌污染情况较严重,菌株对抗生素的耐药率及多重耐药相对较低,对季铵盐类消毒剂MIC较高,消毒剂耐药基因检出率较高,应引起足够重视加强对其的检测.
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