【摘 要】
：
Objective: The expression levels of TGF-β1, receptor (TβR Ⅰ,TβR Ⅱ), Smad protein, P53, MMP-2, Fas, and FasL in actinic keratosis (AK) were investigated.Their correlations were also determined to gain
【机 构】
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The First Department of Medical Oncology,Tumor Hospital of Yunnan Province,The Third Affiliated Hosp
【出 处】
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22nd Asia Pacific Cancer Conference(第22届亚太抗癌大会)
论文部分内容阅读
Objective: The expression levels of TGF-β1, receptor (TβR Ⅰ,TβR Ⅱ), Smad protein, P53, MMP-2, Fas, and FasL in actinic keratosis (AK) were investigated.Their correlations were also determined to gain insight into the mechanism of aberrant TGFβ/Smad signaling and tumorous hyperplasia in AK.Methods: Real-time quantitative polymerase chain reaction(real-time RT-PCR) and MaxVision TM IHC were utilized to detect the expression levels and localization of TGF-β1, TβRⅠ, TβR Ⅱ, Smad-related proteins, P53, MMP-2, Fas, and FasL mRNA and protein in AK, squamous cell carcinoma (SCC),and control normal facial skin paraffin specimens.Then, the digestion method of separation was performed on primary cultured keratinocytes and fibroblasts of AK lesions and normal facial skin.After the cells were identified, real-time RT-PCR and western blot were utilized to detect the expression levels of TGF-β1, TβRⅠ, TβR Ⅱ, Smad related proteins, P53, MMP-2, Fas,and FasL mRNA and protein in keratinocytes and fibroblasts of AK and control cells.
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