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Photoluminescent graphene quantum dots(GQDs)with large surface area and superior mechanical flexibility exhibit fascinating optical and electronic properties,thus providing great promising applications in biomedical engineering.Here,a multifunctional nanoprobe of polyethylene glycol(PEG)/poly(L-lactide)(PLA)-grafted GQDs(f-GQDs)was proposed for simultaneous cell imaging,mixed gene probes delivery and intracellular molecular regulation.The functionalization of GQDs with PEG/PLA imparts the nanocomposite with excellent biocompatibility,low cytotoxicity,superb physiological stability and enhanced photoluminescence among a wide pH range,which is vital for cell imaging.It demonstrated good performance for cell imaging,gene probe delivery and intracellular miRNA analysis.The available large specific surface was used to load mixed gene probes,targeting miRNA and survivin,for gene therapeutic efficacy.This shows higher anti-cancer activity compared to f-GQDs loaded with single gene probe.Considering the good biocompatibility,low cytotoxicity,intrinsic optical properties and large specific surface area for combined loading,the proposed NGO-based nanocarrier is a promising material and may find widespread biological and medical applications.