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Objective: The aim of this study was to explore the molecular mechanisms involved in aerobic exercise ameliorating insulin resistance (IR) by identifying the differentially expressed proteins in skeletal muscle of mice after 6 weeks aerobic exercise with proteome analysis.
Methods: Eighty male C57BL/6 mice were randomly divided into normal feed group and IR model group, fed by normal feed and high-fat-diet respectively for 10 weeks. After 10 weeks, the mice from IR model group were confirmed IR. Then IR model group were randomly divided into high-fat-diet control group (HC) and high-fat-diet exercise group (HE). HE group was required to run on a treadmill for 6 weeks with the intensity of 75%VO2max. After completion of 6 weeks exercise, fasting serum insulin level (FIN) and oral glucose tolerance test (OGTT) were used to assess the effect of 6 weeks aerobic exercise. Total proteins extracted from quadriceps femoris were separated by two-dimensional gel electrophoresis (2-DE ) and the differentially expressed proteins were analyzed using ImageMaster 2D Platinum V5.0, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Tandem Mass Spectrometry (LC/LC-MS/MS).
Methods: Eighty male C57BL/6 mice were randomly divided into normal feed group and IR model group, fed by normal feed and high-fat-diet respectively for 10 weeks. After 10 weeks, the mice from IR model group were confirmed IR. Then IR model group were randomly divided into high-fat-diet control group (HC) and high-fat-diet exercise group (HE). HE group was required to run on a treadmill for 6 weeks with the intensity of 75%VO2max. After completion of 6 weeks exercise, fasting serum insulin level (FIN) and oral glucose tolerance test (OGTT) were used to assess the effect of 6 weeks aerobic exercise. Total proteins extracted from quadriceps femoris were separated by two-dimensional gel electrophoresis (2-DE ) and the differentially expressed proteins were analyzed using ImageMaster 2D Platinum V5.0, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Tandem Mass Spectrometry (LC/LC-MS/MS).