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通过单因素料液比、提取温度、提取时间和提取次数对提取率的影响和正交实验设计方法对绞股蓝多糖水提醇沉法的工艺进行了优化及绞股蓝多糖咀嚼片研制。实验结果表明,水提醇沉法提取多糖最佳工艺条件为料液比1:16、100℃提取2次、每次1h,绞股蓝多糖咀嚼片最佳配方为绞股蓝多糖粉20.00%、淀粉9.75%、蔗糖粉40.00%、奶粉12.30%、甘露醇14.95%、柠檬酸0.50%。
The effects of single factor ratio, extraction temperature, extraction time and extraction times on extraction rate and orthogonal experimental design method were optimized and the preparation of Gynostemma polysaccharide chewable tablets was optimized. The experimental results showed that the optimal extraction conditions of polysaccharides by water extraction and alcohol precipitation were as follows: the ratio of solid to liquid was 1: 16, the extraction time was 2 at 100 ℃ for 1 hour, the optimal formula of Gynostemma polysaccharide chewable tablets was 20.00% of Gynostemma polysaccharide powder, 9.75% 40.00% of sucrose powder, 12.30% of milk powder, 14.95% of mannitol and 0.50% of citric acid.