【摘 要】
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Objective △Np63,the main isomer of p63,has been found to be overexpressed in head and neck squamous cell carcinoma(HNSCC) and other squamous epithelium-derived tumors.This research investigates the fu
【机 构】
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Department of Cell Biology,Harbin Medical University,Harbin,150081,China
【出 处】
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中华医学会医学细胞生物学分会第三届全国学术会议
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Objective △Np63,the main isomer of p63,has been found to be overexpressed in head and neck squamous cell carcinoma(HNSCC) and other squamous epithelium-derived tumors.This research investigates the functional role of △Np63 in promoting cell proliferation of laryngeal squamous cell carcinoma (LSCC),which provides molecular basis for further understanding the role of △Np63 in LSCC occurrence and development.Methods In this study, we use a △Np63 overexpressing cell line to explore the possible roles of △Np63 in cell proliferation and cell-cycle regulation.30%~50% confluent cells were transfected with different siRNA depending on the purpose of experiment.At the designated time after transfection, the cells were harvested and used, respectively,for RT-PCR, Western blot,immunohistochemistry, cell-cycle analysis, cell growth assay and detecting changes of cell-cycle-related gene protein expression.Results △Np63 is highly expressed in LSCC FaDu cell line located in the nucleus.We found that the proliferation of FaDu cells were greatly suppressed with their cell cycles mainly arrested in G1 phase,when △Np63 was silenced by specific △Np63 siRNA or all p63siRNA.Further studies show that △Np63 silencing results in the up-regulation of CKIs,including p21 and p27 in the protein level,and the increased expression of p53.Conclusion △Np63 inhibits the expression of p21 ,p27 and p53 to promote the proliferation of cancer cells, playing a role in maintaining cell proliferation of tumor.
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