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The trypsin inhibitor activities (TIA) of various potato cultivars and processed potatoes were evaluated by measuring the inhibition of trypsin inhibitor activity using N-benzoyl-DL-arginine-p-nitro-anilide (BAPNA) as substrate.The TIA values of five potato cultivars (1.99 to 2.88 mg/g) were significantly different among cultivars (p<0.05).When the TIA values of commercially processed potatoes were determined,no TIA was detected.During cooking,the IT50 (time required to reach 50% inhibition of TIA) values were decreased as heating temperature and time increased.