Rhein attenuates blood-brain barrier disruption after traumatic brain injury similar to rhubarb by s

• 作 者: 王杨 范荣 张春虎 夏子安 熊新贵 彭伟军 黄卫 罗杰坤 唐涛 黄熙
• 出 处: 第十一届全国中西医结合基础理论学术研讨会
• 发表日期: 2015年9期

　　Objective: Traumatic brain injury (TBI) is considered a silent epidemic, as society is largely unaware of the magnitude of this problem.The present study aimed to determine whether the antioxidative herbal medicine rhein exerted BBB protection similar to rhubarb following TBI by suppressing MMP-9 expression via inhibition of NADPH oxidase/NF-kappa B pathway.Methods: UPLC-MS/MS was applied to determine the amounts of rhein from rhubarb decoction.Sprague-Dawley rats induced by Controlled Cortical Impact gave rise to acute oxidative response.Treatment groups were administered with rhubarb (3 g/kg, 6 g/kg and 12 g/kg) and its absorbed compound rhein (12 mg/kg), vehicle group and sham group were gavaged with equal saline.The assays of Evans blue extravasation and brain water content were assessed at 6th, 12th and 24th hour after TBI.The levers of gp91phox, MMP-9 and NF-kappa B (p65) were measured at 6th, 12th and 24th hour by RT-PCR, immunohistochemistry and western blot assay.Results: The content (mg/g) of rhein in rhubarb was 1.024±0.076.The results indicated that rhubarb and rhein significantly attenuated BBB permeability and brain edema.The significant downregulation of gp91phox, MMP-9 and NF-kappa B (p65) mRNA levels and protein expressions in brain tissues was observed after rhubarb and rhein administration.Conclusions: Rhein attenuates blood-brain barrier disruption after traumatic brain injury similar to rhubarb by suppressing MMP-9 expression though inhibition of NADPH oxidase/NF-kappa B pathway.Rhubarb and its key absorbed compound rhein had potential therapeutic implications for BBB impairment of TBI.