A Novel Polyacrylamide Gel Electrophoresis for Rapid and High Resolution Separation of Peptides

• 作 者: ZhengLiu;HaiyangXie;HuaXiao;LiuyinFan;ChengxiCao;
• 机 构: Laboratory of Bio-separation and Analytical Biochemistry,State Key Laboratory of Microbial Metabolis
• 出 处: 第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
• 发表日期: 2014年期

　　Hydrogels have many applications,ranging from tissue engineering to protein separation.Most hydrogels do not exhibit hight resolution,for example,a polyacrylamide hydrogel used to separate proteins,the resolution of proteins decided by the concentrations of acrylamide.The high concentrations means fragile.However,we cannot obtained a satisfaction for the resolution of proteins smaller than 30 kDa.Herein we show that a complex hydrogel via compolymerization.The complex hydrogel exhit better mechanical behavior and better resolution.The separation process can be completed in 3-4 h,and the protocol for separation small molecular weight proteins completed in 1-2 d usually.Hydrogel confirmed by fourier transform infrared spectrometer,electron microscopy,1H NMR spectroscopy and rheology.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)tests indicate good resolution for small molecular weight proteins,which suggest potential separation applications.