【摘 要】
：
DNA topoisomerase Ⅱ (topo Ⅱ) cleaves both strands of a duplex DNA, allows another duplex to pass through the gap, and finally re-seals the gap.Analyses of developing rat cerebellum have revealed that
【机 构】
：
Department of Genome Dynamics Okayama University Japan
【出 处】
：
BIT`s 3rd Annual World Congress of NeuroTalk-2012(2012第三届国际神
论文部分内容阅读
DNA topoisomerase Ⅱ (topo Ⅱ) cleaves both strands of a duplex DNA, allows another duplex to pass through the gap, and finally re-seals the gap.Analyses of developing rat cerebellum have revealed that isozyme beta (topo Ⅱbeta) is highly expressed in differentiating cerebellar neurons, both Purkinje cells and granule cells, after the final cell division.To study the role of topo Ⅱbeta in differentiating neurons, we used primary culture of rat cerebellar granule neurons that can mimic the in vivo situation.Gene expression analysis on microarray revealed that topo Ⅱbeta is essential for the transcriptional activation of a subset of genes that frequently locate right next to long and AT-rich intergenic region (thus termed LA gene).We also mapped the topo Ⅱbeta-acting sites (toposites) by a novel functional ChIP technology called eTIP.Genomic positions of LA gene and toposites suggested that distal strand passage events (DSP) catalyzed by topo Ⅱbeta may activate the LA gene transcription.To locate the DSP sites, we are now developing a method termed eTIP-PES (Paired End Sequencing).The DNA fragments on topo Ⅱbeta-DNA complex were first ligated to biotinylated adaptors and ligated again through the adaptor to form chimeric DNA fragments.After selection with streptavidin beads, chimeras were processed for paired-end sequencing on a new generation sequencer.The results suggested the occurrence of distal genomic interactions mediated by topo Ⅱbeta.
其他文献
Aging is associated with a general decline of physiological functions, and those which depend on the central nervous system, such as sexual behavior are the most affected.The critical age at which an
Epidermal growth factor (EGF) and neuregulin-1 are the ErbB receptor ligands that are implicated in schizophrenia neuropathology and neurotrophic regulation of midbrain dopaminergic neurons.mRNA for E
The immediate early gene, Arc (activity-regulated cytoskeletal-associated protein), regulates multiple forms of protein synthesis-dependent synaptic plasticity, including LTP, LTD, and homeostatic pla
Damage to peripheral nerves often results in various sensory and motor abnormalities.The loss of neurotrophic factor support has been proposed to contribute to the development of peripheral neuropathy
At neuromuscular synapses, agrin stabilizes postsynaptic acetylcholine receptor (AChR) clusters by signaling through the Muscle Specific Kinase (MuSK) complex.Imaging of cultured myotubes showed that
Brain plasticity refers brains ability to change according to physiological and pathological life events.Many different mechanisms participate to brain plasticity, including genesis of new neurons and
In Drosophila, Mushroom Bodies (MBs) are the site of CS-US association for olfactory memory.MBs receive olfactory CS inputs from cholinergic projection neurons and dopamine (DA) neurons convey the ave
Intent conditions influence human behavior, and the movement of the eye is used by humans to evaluate one anothers reasoning and detecting intent state.The purpose of this study was to investigate the
Values of global and regional cerebral blood flow, received in experimental and clinical investigation as the informative criteria for brain blood supply, are often misleading.Although it is known, th
Introduction: Bipolar disorder is a severe mental illness characterized by alternating bouts of mania and depression.The pharmacotherapy of bipolar disorder includes a variety of drugs, however, nearl