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In order to discover potential glycoprotein biomarkers in colon cancer, we used microarray and MS analysis approach.In this study, we first used lectin microarray to investigate the glycoprotein profile differences between colon cancer and corresponding adjacent tissue, and Solanum Tuberosum lectin(STL)-binding glycans were found to be elevated in colon cancer, indicating an elevation of GlcNAc level, which was then confirmed by lectin histochemistry.The glycoproteins that STL binds were then identified by LCMS/MS.Here, we used selected lectin-STL to perform a targeted glycoptoteomic analysis of colon cancer and adjacent tissues, and our results have identified several glycoproteins that display tumor-specific glycosylation changes, of which we selected HSP90β and Annexin A1 to carry out the further study.Verification was then performed using immunoprecipitation followed by lectin blot detection, tissue microarray and western blot.Results showed both HSP90β and Annexin A1 were GlcNAcylated, and they were elevated in colon cancer,while compared with adjacent control.Our finding suggested that data obtained from two of these glycoprotein markers-HSP90β and Annexin A1, have confirmed that tumor-specific glycan changes can be used to distinguish colon cancer patient from normal, and they can be the candidate biomarkers of colon cancer.