【摘 要】
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Background: Fat tissue graft has been commonly used for soft-tissue augmentation.However, the mechanisms underlying the maintenance of graft volume and weight are still unclear.As morphological featur
【机 构】
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陕西省西安市第四军医大学西京医院整形外科
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Background: Fat tissue graft has been commonly used for soft-tissue augmentation.However, the mechanisms underlying the maintenance of graft volume and weight are still unclear.As morphological features provide direct evidences for cell death and survival, we aimed to investigate the fate of grafted adipocytes and the dynamic changes in the remodeling of adipose tissues by transmission electron microscopy (TEM) technique.Methods: The unilateral inguinal fat pad of C57BI/6J mice was autografted to the dorsa of the mice.Perilipin expression and morphological changes were investigated by immunohistochemistry staining and TEM, respectively, in grafted tissues collected at posttransplantation days 0, 1, 3, 5, 7, 14 and 30.Results: TEM analysis revealed that most adipocytes in grafts showed traits of cell death on post-grafting day 3.Multilocular adipocytes with na?ve nucleiwere observed as early as day 5 and a larger number of multilocular adipocytes were found on day 14.Perilipin immunostaining revealed that only some adipocytes located in the margin of grafts survived through the ischemic injury.New adipocyes were visualized at the periphery of the grafts while the scope of viable adipocyte zonal areas increased from day 5 to day 30.Conclusions: The results provide ultrastructural evidences associated with the remodeling dynamics of adipose tissue grafts.It is suggested that maximized volume of graft should be obtained through promoting regeneration other than improving survival of grafted adipose tissues.
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