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In this paper, the hollow polymer nano-latex microspheres with uniform size and good monodisperse made by in-situ polymerization with the method of encapsulation non-solvent, and the effect of crosslinking monomer (DVB) dosage in the shell on the morphology and opacity of the hollow polymer nano-latex microspheres were investigated by the characterization methods of particle size analysismeter, viscosity meter, acid value analysis, SEM, TEM and UV-Vis spectrophotometry.