A Rapid Three-Dimensional Microfluidic Mixer for Tracking Early Folding Kinetics of G-Quadruplex und

• 作 者: ChaoLiu;XiaojunFeng;Bi-FengLiu;
• 机 构: Systems Biology Theme,Department of Biomedical Engineering,College of Life Science and Technology,Hu
• 出 处: 第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
• 发表日期: 2004年期

　　Rapid mixing of high viscosity solutions is crucial for tracking the folding kinetics of macromolecules under molecular crowding conditions.In this paper,we design a three dimensional micromixer for rapid mixing of high viscosity fluids.Numerical simulations and experimental characterizations are conducted.At a sample flow rate of 0.21 mL/min,the micromixer could achieve a mixing time of 120 μs for high viscosity solutions.In comparison to two-dimensional mixers previously reported,the three-dimensional micromixer offers unique advantages for its short mixing time,less sample consumption.We further used this micromixer to track the early folding kinetics of human telomere G-quadruplex under molecular crowding conditions.