芝麻香型酒高温大曲细菌多样性及高温放线菌新种的研究

来源 :第七届全国微生物资源学术暨国际微生物系统与分类学研讨会 | 被引量 : 0次 | 上传用户:lostwing56
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  采用可培养分析技术结合非培养分析技术,对芝麻香型白酒高温大曲制曲过程的细菌菌群进行动态跟踪,系统分析了高温大曲发酵过程细菌多样性及演替变化规律,建立了高温大曲优势细菌动态评价模型,提出了高温放线菌在制曲过程中的优势地位和重要性;从传统白酒高温大曲中分离获得了高温放线菌新菌种CICC 10681T Thermoactinomyces daqus sp.nov.,补充和丰富了高温放线菌属(Thermoactinomyces sp.)菌种种类,同时该菌株具有一定的高温蛋白酶活性,对于探究芝麻香型白酒的产香机理具有重要研究意义;分离、筛选和鉴定了高温大曲嗜热功能菌,其中CICC 15073 Bacillus subtilis subsp.subtili具有较高的中性蛋白酶活力,已应用于高温制曲过程的微生物强化;通过筛选和功能性评价,获得近百株产蛋白酶和酯酶等白酒酿造功能菌株,构建了白酒酿造微生物菌种资源库,应用于芝麻香白酒优质酒酿造过程,对提升传统白酒酿造工艺精准控制能力具有重要意义.
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