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Sulforaphene (4-methylsulfinyl-3-butenyl isothiocyanate),which has significant chemopreventive activities,is an important phytochemical ingredient produced by myrosinase hydrolysis of glucoraphenin (4-methylsulfinyl-3-butenyl glucosinolate) found in radish seeds.In our previous research,sulforaphene was successfully isolated and purified by macroporous resin and preparation HPLC or by high-speed countercurrent chromatography [1,2].In further study,we found that sulforaphene was unstable and converted rapidly to a water soluble degradation product in the hydrolytic process.The degradation product was successfully purified by preparative high-performance liquid chromatography on a C18 column using 10% methanol in water as the mobile phase.Based on MS and NMR spectroscopy data,the degradation product was identified to be 6-[(methylsulfinyl) methyl]-1,3-thiazinan-2-thione.In order to inhibit the degradation of sulforaphene,the pH value of the hydrolysis system was first kept at 7.0 for 20 min and then adjusted immediately to 2.0 after the concentration of sulforaphene reached its maximum.Through controlling the pH value of the hydrolysis system,the yield of sulforaphene increased 21.6 times compared with that of the control.