新型MIP-2γ/GM-CSF融合蛋白对DC的双重生物学功能及相关机制研究

来源 :中国免疫学会第十届全国免疫学学术大会 | 被引量 : 0次 | 上传用户：zhaodaxiang

【摘要】

：

　　目的：制备新型MIP-2γ/GM-CSF细胞因子融合蛋白，研究其趋化和激活DC的双重生物学功能.方法：通过基因工程方法构建高表达MIP-2γ/GM-CSF融合蛋白的2种P.pastoris酵母工程菌株

【作者】

：

张意[1]陈国友[1]王建莉[2]于益芝[1]蒋应明[1]周维明[1]曹雪涛[1]

【机构】

：

第二军医大学免疫学教研室暨医学免疫学国家重点实验室

【出处】

：

中国免疫学会第十届全国免疫学学术大会

【发表日期】

：

2015年期

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　　目的：制备新型MIP-2γ/GM-CSF细胞因子融合蛋白，研究其趋化和激活DC的双重生物学功能.方法：通过基因工程方法构建高表达MIP-2γ/GM-CSF融合蛋白的2种P.pastoris酵母工程菌株，并优化发酵、纯化条件，制备了高纯度的MIP-2g/hGM-CSF、MIP-2g/mGM-CSF融合蛋白.首先以Transwell体系检测融合蛋白对树突状细胞DC的诱导迁移效应.接着以流式细胞仪分析重组蛋白对DC表型的影响，检测其作用DC后引起的MLR反应和细胞因子分泌情况.进行动物体内实验，研究MIP-2γ/GM-CSF融合蛋白对CT26细胞转染的荷瘤小鼠的抗肿瘤效应，并研究了其相关的作用机制.

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新型MIP-2γ/GM-CSF融合蛋白对DC的双重生物学功能及相关机制研究

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