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Fungal plant pathogens produce proteinaceous effectors that can increase their pathogenicity.These fungal effectors are frequently small secreted proteins with limited phylogenetic distribution.Using a proteomics approach for analyzing the secretome is a promising strategy for the identification of effector proteins of phytopathogenic fungi.However, the primary limitation of this strategy is the low identification rate of spectrometry-based proteomic methods that rely heavily on the accuracy and integrity of the reference sequence database.