Rhein reduces fat weight in db/db mouse and prevents diet-induced obesity in C57BL/6 mouse through t

• 作 者: YuZhang[1]ShengjieFan[1]NaHu[2]MingGu[1]ChunxiaoChu[1]YimingLi[1]XiongLu[2]ChengHuang[1]
• 机 构: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
• 出 处: 2014年上海市研究生学术论坛:中医药的协同创新
• 发表日期: 2014年期

　　Rheum palmatum has been used most frequently in the weight-reducing formulae in traditional Chinese medicine.However, the components of rheum palmatum that play the anti-obesity role are still uncertain.Here, we tested the weight-reducing effect of two major rheum palmatum compounds on db/db mouse.We found that rhein (100 mg kg-1day-1), but not emodin, reduced the fat weight in db/db mouse.Using diet-induced obese (DIO) C57BL/6 mice, we identified that rhein blocked high-fat diet-induced obesity, decreased fat mass and the size of white and brown adipocytes, lowered serum cholesterol, LDL-cholesterol and fasting blood glucose levels in the mice.To elucidate the underlying mechanisms, we used reporter assay and gene expression analysis, and found that rhein inhibited peroxisome proliferator-activated receptor γ(PPARγ) transactivity and the expression of its target genes,suggesting that rhein may act as a PPARγ antagonist.Our data indicate that rhein maybe a promising choice for anti-obesity therapy.