Experiment of transplanting Metanephric mesenchymal transfected by uPA gene to therapy chronic kidne

• 作 者: 毕凌云 易著文 曹艳 黄丹琳 何庆南 何小解 莫双红
• 机 构: 中南大学湘雅二医院小儿肾脏病研究室 410011
• 出 处: 中华医学会第五次全国儿科中青年学术交流大会
• 发表日期: 2008年3期
• 关键词: chronic kidney disease uPA gene transfecte mesenchymal

Study regeneration role and possible mechanism of metanephric mesenchymal and metanephric mesenchymal transfected by uPA gene in CKD.Method：1. Construction and package of recombinant plasmid of uPA (urokinase-type plasminogen activator) gene and pAAV-IRES- hrGFP vector.2. The viral particles of rAAV and AAV were transfected into rats metanephric mesenchymal (rat-inducible metanephric mesenchymal-18, RIMM-18) in vitro and the positive RIMM-18 cells that expressed GFP were detected using the inverted fluorescence microscope.3.Study regeneration role and possible mechanism of metanephric mesenchymal and metanephric mesenchymal transfected by uPA gene in CKD that were transplanted via caudal vein.Conclution：1.The recombinant plasmid of AAV and uPA gene were successfully constructed andpackaged. 2.The transfectian rate of rAAV and AAV viral particles to RIMM-18 was as high as 60％. AAV vectorcan deliver uPA gene effectively.