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Here,time-decoupled comprehensive two-dimensional ultra-high liquid chromatography(UPLC)coupled with an ion mobility(IM)-high resolution mass spectrometer(HRMS)was established and used to analyze ginsenosides from the main roots of white ginseng(WG)and red ginseng(RG),which enabled the separation of complex samples in four dimensions(2D-LC,ion mobility,and mass spectrometry).