The gypsy Insulator of Drosophila melanogaster, Together With Its Binding Protein Suppressor of Hair

• 作 者: WenjingShe;JunhuiWang;WeiqiangLin;YubinZhu;YongChen;WeiyuanJin;YanjunYang;NingHan;HongwuBian;MuyuanZhu;
• 机 构: Department of Biotechnology, College of Life Sciences, Zhejiang University, Zijingang Campus, Hangzh
• 出 处: 2010中国青年遗传学家论坛
• 发表日期: 2010年期

　　The variation of expression pattern exhibited by a transgene as a result of random integration, known as position effect, is, among other mechanisms, a particular challenge to reverse genetics.We present a strategy to counteract position effect in Arabidopsis thaliana by flanking the transgenes with the gypsy insulator from Drosophila melanogaster.In addition, Suppressor of Hairy-wing [Su(Hw)], the binding protein of the gypsy insulator, was coexpressed.Results indicated that the gypsy insulators could efficiently improve the expression levels of reporter genes driven by various kinds of promoters by 8-to 13-fold.Coexpression of the Su(Hw) protein led to a more uniform expression level of transgenes, as the coefficient of variation of expression levels was reduced further.